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Eurofins
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ATCC
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Macrogen
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ATCC
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Illumina Inc
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Illumina Inc
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Journal: bioRxiv
Article Title: Methanobacterium nebraskense sp. nov., a hydrogenotrophic methanogen isolated from saline wetland soil
doi: 10.1101/2025.11.18.688885
Figure Lengend Snippet: Maximum likelihood phylogenetic tree based on 16S rRNA gene sequences (1326 bp) showing the position of strain ACI-7 T and type strains of the genus Methanobacterium . Numbers at nodes indicate bootstrap percentages based on 1000 replicates; only values ≥ 50% are shown. Accession numbers are indicated in brackets. Methanothermus fervidus V24S T [NR102926.1] was used as an outgroup (not shown). Bar, 0.03 substitutions per nucleotide position.
Article Snippet: The 16S rRNA gene was PCR amplified from strain ACI-7 T using the primers A109F ( ) and Met1340R ( ) and sequenced by
Techniques:
Journal: Frontiers in Veterinary Science
Article Title: Application of next-generation sequencing for detecting Mycoplasma contamination in veterinary vaccines
doi: 10.3389/fvets.2025.1657098
Figure Lengend Snippet: Sequence alignment of the universal Mycoplasma forward (Myco_F) and reverse (Myco_R) primers with the 16S rRNA sequence of E. rhusiopathiae (GenBank accession no. NR_040837 ). For each primer, the upper line represents the primer sequence and the lower line represents the aligned E. rhusiopathiae sequence. Identical nucleotides are indicated by dots (“.”) in the lower sequence, while mismatches are shown as their respective bases and highlighted in pink to indicate regions of divergence that may result in non-specific binding. The forward primer aligns to positions 95–115 bp, and the reverse primer aligns to positions 534–555 bp of the E. rhusiopathiae 16S rRNA gene. This alignment highlights the potential for cross-reactivity that could generate false positives in PCR assays targeting Mycoplasma spp.
Article Snippet: For E. rhusiopathiae , the
Techniques: Sequencing, Binding Assay